Prof. Masoumeh Ghalkhani
Shahid Rajaee Teacher Training University, Iran

Biography:

Medetomidine, 4-[1-(2,3-dimethylphenyl) ethyl]-1himidazole hydrochloride, is a synthetic drug that is prescribed as a surgical anesthetic and sedative. This drug has analgesic effects too. In this work, a screen-printed carbon electrode (SPCE) was modified by a composite of N and P-doped carbon nano-onions (N, P CNO), MoS2, and poly (melamine) (PME) (SPCE/N, P CNO/MoS2/PME). First, synthesized nanomaterials and fabricated electrodes were evaluated and analyzed by FT-IR, FE-SEM, and XRD techniques. Then, the voltammetric response of medetomidine was evaluated using SPCE/N, P CNO/MoS2/PME. The effect of N, P CNO, MoS2, and melamine, deposited electrochemically on the surface of SPCE, was evaluated for medetomidine measurement. Results proved that the mentioned nanomaterials enhanced the SPCE surface area and facilitated the electron transfer rate. A cathodic peak appeared in a buffered solution with pH = 7.0 containing medetomidine. A diffusion-controlled process resulted in the medetomidine electroreduction at SPCE/N, P CNO/MoS2/PME. The synergetic effect of N, P CNO, and MoS2, providing wide active surface area, and PME as an electron conduction booster enhanced the modified electrode response toward medetomidine. The calculated detection limit for medetomidine in the concentrations range of 0.04 to 700 μM was 10 nM. The SPCE/N, P CNO/MoS2/PME was able to determine accurately the medetomidine concentration in mouse blood samples with 90-98.2% recovery. The consecutive analysis of a medetomidine solution led to outstanding repeatability with RSD = 4.10%. The calculated RSD = 3.54% for the reproducibility test of SPCE/N, P CNO/MoS2/PME preparation introduced it as a proper sensor. The long-term stability is another outstanding feature of the SPCE/N, P CNO/MoS2/PME, for which a 23% decrement of the peak current resulted after 6 months of storage.
Keywords: Medetomidine, Carbon nano anions, MoS2, Melamine, N doped, P doped