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Associate Professor  Lalit  M. Pandey
Department of Biosciences and Bioengineering, IIT Guwahati,  India

Title: Surface engineering of Ti6Al4V by forming hybrid self-assembled monolayers and its effect on collagen-I adsorption, osteoblast adhesion and integrin expression


This study focused on the surface engineering of Ti6Al4V by forming self-assembled monolayers (SAMs) towards improving bio-interfaces interactions [1]. Adsorption of adhesive protein collagen-I (Col-I) and subsequently on osteoblast adhesion and integrin expression were studied on the engineered surfaces. The moderate hydrophobic Ti6Al4V surfaces (θ = 78 ± 3°) were prepared by forming hybrid self-assembled monolayers (SAMs), which contain both hydrophobic and hydrophilic moieties on the same SAM molecule. The experimental adsorbed amounts of Col-1 were compared with the predicted ones using the Gibbs equation, which were overestimated but linearly related. Secondary structure and morphology analyses of adsorbed Col-I revealed globular morphology with significant change only in helical content. Cell-surface interactions on hybrid surface pre-adsorbed with Col-1 exhibited better cell adhesion (~100%) and spreading area (1127 µm2) as compared to cells adhered to hybrid surface without (adhesion of 69% and cell area of 509 µm2) and with (adhesion of 94% and cell area of 908 µm2) fetal bovine serum (FBS) in cell culture media. It was observed that higher expression of α1 and α2 integrins on surfaces with pre-adsorbed Col-I and were correlated with the increase in nuclei area indicating α1 and α2 mediated cell adhesion promoted the cell proliferation. Overall, cell-surface interactions were improved on hybrid surfaces with pre-adsorbed Col-I, which designated the surface engineered Ti6Al4V as potential implant biomaterials.

1. Hasan, A. and L.M. Pandey, Surface modification of Ti6Al4V by forming hybrid self-assembled monolayers and its effect on collagen-I adsorption, osteoblast adhesion and integrin expression. Applied Surface Science, 2020. 505: p. 144611.


Dr. Lalit M. Pandey is an Associate Professor in the Department of Biosciences and Bioengineering, IIT Guwahati. He has completed his Ph.D. in Chemical Engineering from Indian Institute of Technology, Delhi. He was awarded Erasmus Mundus India4EU fellowship to pursue his research at Laboratoire des Matériaux et du Génie Physique (LMGP), Grenoble-INP, France for 18 months during 2010 to 2012. Prior to joining IIT Guwahati, he was working as a Scientist with the Central Pollution Control Board, Ministry of Environment & Forests, Govt. of India from 2009 to 2014 and was involved in research/study relating to water and air pollution in agrobased industries. He has received Shastri Indo-Canadian award 2020, DST-UKIERI award 2018, IEI (The Institution of Engineers [India]) Young Engineers Award 2017, Innovation in Science Pursuit for Inspired Research (INSPIRE) Faculty Award 2014 and Early Career Research Award from Science and Engineering Research Board (SERB), Govt. of India. 
He has worked in the area of Chemical & Biochemical engineering including Surface and Interfacial Science (Bio-interfaces and Biomaterials, Protein’s structure and its stability), Nano and Biotechnology, and Environment Chemical Engineering. He has published 67+ scientific articles in international journals. He has co-authored 53+ conference papers and 18+ book chapters. He has co-edited one book titled “Biointerface Engineering: Prospects in Medical Diagnostics and Drug Delivery” published by Springer Nature in 2020. He is on the Editorial Board of five international journals and Guest Associate Editor in Biomaterials (Frontiers).

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