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Biography

Prof.  Beilei  Wu
Institute of Plant Protection, Chinese Academy of Agricultural Sciences (CAAS),  China

Title: Effects on the Biocontrol functions of Trichoderma spp. by the mycoviruses

Abstract:

 Trichoderma spp. are known to impact host plant growth and defense activities. With the findings of the mycoviruses from Trichoderma spp., more and more effects on the sporogenesis and biocontrol functions of host induced by mycoviruses were known. In our research,  Trichoderma harzianum mycovirus 1 (ThMV1) from T. harzianum strain (T525) not only affected the phenotype of the host strain but also reduces its biocontrol function on F. oxysporum f. sp. cucumerinum. Using the yeast two hybrid nuclear system to screen, 57 proteins involving the interaction between T525 and ThMV1 have been obtained, among which 40 proteins interacted with ThMV1-RdRP, 29 proteins interacted with ThMV1-CP and 12 proteins interacted with both ThMV1-RdRP and ThMV1-CP. For the hexokinase1 (HXK) also was the interacted protein with ThMV1-CP, the hexokinase gene (hxk) knockout mutant (T525-hxk) and reverse mutant (T525Rhxk) of T525 were obtained. connecting the results of transcriptomic analysis, Real-time RT-PCR and the phenotype of cucumber, the interaction of ThMV1-CP/HXK in T525 was proved to negatively regulate the disease resistance pathway of T525 and positively regulate the growth promotion effect of T525 in the cucumber. 

 

Biography:

RESEARCH INTERESTS
1. Collection, diversity and evaluation of Trichoderma spp.
2. Investigation on fermentation process for Trichoderma chlamydospore production
and industrialization
3. Identification and evaluation of Mycovirus from Trichoderma. spp.
4. Interactions among Trichoderma spp., pathogen and plant
5. Collection resource and evaluation on endophytes from plant
6. The molecular epidemiology, evolution and fitness of Alfalfa mosaic virus (AMV)
population in host range and different environments.
7. The molecular epidemiology and evolution of plant virus population;
8. The assessment of epistasis level in the evolution of virus population and the effect
on the adaptation, robustness of the population;
9. The discrepancy of fitness for the virus population in host range and different
environments;
10. The suppressor of the virus;
11. Transgenic wheat and rice resistant to plant virus mediated by RNAi

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