Dr.  Mauro  Luisetto
Previous Europeans specialist laboratory medicine EC4 REGISTERED,  Italy

Title: m R.N.A. purification : TECHNOLOGY ASPECTS and impurities TFF, Chromatography, UF/DF ( Resins, magnetic beads , monoliths )

Abstract:

m R.N.A. VACCINE production like other biopharmaceutical need various purification stages in the manifacturing  process.

In this process are used technique like : T.F.F., tangential flow filtration  followed by different chromatografic procedure ( affinity and ion exchange separation )  with various kinds of resin an then UF/DF technique. ( ultrafiltration -diafiltration).

So it is of interest to verify the material used for this kind of  procedure as solid phases  or for the membrane and if released impurity in the final product.

 For Silica-based Reversed-Phase packings, a carbon load percentage indicates the amount of functional bonded phase attached to the Silica-base material.

Aim of this work is to investigate the role played by this characteristics in the separation process of  m R.N.A.

Because  this  parameter  influence the  retention time it is interesting to evaluate the use in separation technique of biophramaceutictals.

And this also for the  carbon coated silica colums .

Silica gel for chromatography can be produced by systetic process bu alsto form Rice treated at high temperature .

What kind of effect can be played using an high level of carbon coated silica material on the final purified product ?

The graphitic  particle can be finded as impurity during the manifacturing process of this resins ?

And waht is the role played by carbon  ( graphene- quantum dots) membrane  as reported in various research application?

Biography:

Applied pharmacologist, hospital pharmacist manager, professorship general toxicology

Professor of environmental toxicology and pharmacology, Previous Europeans specialist laboratory medicine EC4 REGISTERED since 2019.

Many publications and books in scientific discipline : pharmacology , pharmacy , toxicology, pathology , neuroscience, virology , epidemiology, management , ICT

RESEARCH INTEREST  SCORE ON RESEARCHGATE  : MORE THEN 99% OF ALL RG MEMBERS

IMA academy Marijnskaya Italy  Applied chemistry and industrial chemistry branch.