Prof.  Marc  Baaden
Institut de Biologie Physico-Chimique,  France

Title: High fidelity ryanodine receptor reconstruction from cryo-EM data

Abstract:

Advances in Biochemistry and Molecular Biology, in particular integrative modeling techniques, have ushered in a new era of understanding large macromolecular assemblies. Unraveling the intricate dynamics and mechanisms underlying their biological functions requires a careful synthesis of all available data. To achieve this, we have developed a modeling pipeline that seamlessly combines automated processes with hands-on, human-guided expertise. In this presentation, I illustrate our methodology using the ryanodine receptor, an ion channel that controls ion flux across the cell membrane and deftly switches between open and closed states. These transitions require subtle conformational changes within the receptor, which is a remarkable feat given the considerable size of the system. The challenge is to consistently track these states using information from cryo-electron microscopic structures. We are focusing on interactive, immersive modeling tools specifically designed to overcome the unique obstacles encountered in this multi-faceted endeavor. With these innovative approaches, we aim to shed new light on the mysteries of macromolecular assemblies and pave the way for a deeper understanding of their biological significance.

Biography:

Marc Baaden (LBT, UPR 9080) is a senior research director (DR1) at CNRS. He is currently head of the CNRS UPR9080 Theoretical Biochemistry Unit. He is co-author of > 120 publications and is known for molecular dynamics studies on membrane proteins published in Nature, PNAS, PLoS Comput Biol... , etc. Trained as a physical chemist, his research focuses on biological systems and phenomena at membranes and interfaces, where water and ion transport play a special role. He is internationally known for interactive molecular design approaches with multi-scale representations using unique in-house software. His research on protein simulations, bioinformatics, and permeating membrane systems features an intrinsically interdisciplinary approach. It combines chemistry, physics, biological sciences, and computer science. In particular establishing links with new technologies such as virtual reality and artificial intelligence. He has received several awards in the past and several of his papers have been recommended by the Faculty of 1000 in Biology and Medicine.

Webpage: http://www.baaden.ibpc.fr