Prof.  Nanguang  Chen
National University of Singapore,  Singapore

Title: Advancing Laser Speckle Imaging for optical sectioning and quantification

Abstract:

Laser speckle imaging (LSI) is a widely used technique to detect the perfusion of blood flow and monitor the development of vascular system. Current laser speckle techniques have limited capability in dealing with 3D vessels distributions, and they are typically used to evaluate the relatively flow changes instead of absolute velocities. Here, we report an innovative laser speckle imaging method by coupling light-sheet microscopy and transmissive microscopy. Intensity autocorrelation analysis, particle image velocimetry (PIV), and other image processing methods have been applied to the raw images to generated tomographic images of blood vasculature, scalar velocity distribution, and vector velocity destruction. The experimental results have demonstrated that our proposed approach could achieve excellent optical sectioning capability and quantification accuracy in zebrafish models.

Biography:

Dr. Nanguang Chen is currently an Associate Professor of Biomedical Engineering at the National University of Singapore (NUS). He received his PhD in Biomedical Engineering in 2000 from Tsinghua University. He also received his MSc in Physics and BSc in Electrical Engineering in 1994 (Peking University), and 1988 (Hunan University), respectively. He joined the Optical and Ultrasound Imaging Lab at the University of Connecticut in 2000 as a postdoctoral fellow and then became an Assistant Research Professor in 2002. Since 2004, he has been a faculty member with NUS. His research interests include diffuse optical tomography, optical coherence tomography, and novel microscopic imaging methods.