Single-molecule nanopore has attracted much attention due to its advantages such as long-read sequencing, ultra-high spatiotemporal resolution, real-time detection, and high speed. Herein, we will report three nanopore stories: (1) the construction of semi-synthetic nanopore protein and its long-reading DNA and protein sequencing; (2) nanopore-based peptide discrimination with single amino acid resolution; (3) high-throughput biochip for ultralow sensing of protein biomarkers. 

Ji, Zhouxiang, Ph.D., is a professor at the School of Life Science and Technology, Xidian University. He also serves as the Director of the International Science and Technology Cooperation Base of Xi'an Intelligent Precision Diagnosis and Treatment, and the Director of the Xidian University Comprehensive Experimental Center for Chemistry and Bioscience. He graduated from Dr. Peixuan Guo's lab at the College of  Pharmacy of The Ohio State University in May 2019. From September 2019 to December 2012, he worked at BGI Research as the department leader of the nanopore project. His recent research focuses on single-molecule nanopore biosensing, including DNA sequencing and proteomics. He has received several fundings, such as the National Natural Science Foundation of China, the Qin Chuangyuan Program, the National Key Research and Development Program, and others.